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原著論文


<1991年>

S. Taguchi, K. Nishiyama, I. Kumagai, H. Momose and K. Miura:
Relationship between Utilization of Dual Translational Initiation Signals and Protein Processing in Streptomyces
Mol Gen. Genet., 226, 328-331 (1991)

I. Hirao, S. Yoshizawa and K. Miura:
Gel Electrophoresis Using a Fluorescence Agent for Analysis and Purification of Non-labeled Synthetic DNA Fragments
Nucleic Acids Res., 19, 4003 (1991)

S. Kojima, Y. Nishiyama, I. Kumagai and K. Miura:
Inhibition of Subtilisin BPN' by Reaction Site P1 Mutants of Streptomyces Subtilisin Inhibitor
J. Biochem., 109, 377-382 (1991)

Y. Kumazawa, H. Himeno, K. Miura and K. Watanabe:
Unilateral Aminoacylation Specificity between Bovine Mitochondria and Eubacteria
J. Biochem., 109, 421-427 (1991)

S. Asakawa, Y. Kumazawa, T. Araki, H. Himeno, K. Miura and K. Watanabe:
Strand-specific Nucleotide Composition Bias in Echinoderm and Vertebrate Mitochondrial Genomes
J. Mol. Evol., 32, 511-520 (1991)

Y. Kumazawa, C.J. Schwartzbach, H.-X. Liao, K. Mizumoto, Y. Kaziro, K. Miura, K. Watanabe and L.L. Spremulli:
Interactions of Bovine Mitochondrial Phenylalanyl-tRNA with Ribosomes and Elongation Factors from Mitochondria and Bacteria
Biochim Biophys. Acta, 1090, 167-172 (1991)

A. Tamura, K. Kanaori, S. Kojima, I. Kumagai, K. Miura and K. Akasaka:
Mechanisms of Temporary Inhibition in Streptomyces Subtilisin Inhibitor Induced by an Amino Acid Substitution, Tryptophan 86 Replaced by Histidine
Biochemistry, 30, 5275-5286 (1991)

Y. Takeuchi, S. Noguchi, Y. Satow, S. Kojima, I. Kumagai, K. Miura, K. T. Nakamura and Y. Mitsui:
Molecular Recognition at the Active Site of Subtilisin BPN': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor SSI (Streptomyces Subtilisin Inhibitor)
Protein Engng., 4, 501-508 (1991)

O. Sakatsume, T. Yamaguchi, M. Ishikawa, I. Hirao, K. Miura and H. Takaku:
Solid Phase Synthesis of Oligoribonucleotides by the Phosphoramidite Approach Using 2'-O-1-(2-Chloroethoxy)ethyl Protection
Tetrahedron Lett., 47, 8717-8728 (1991)

M. Ishikawa, K. Ikebukuro, I. Hirao and K. Miura:
Synthesis of Fused Oligoribonucleotides with Trideoxyribonucleotides Containing Phosphorothioate to Stabilize against Nuclease Activity
Nucleosides nucleotides, 10, 1377-1390 (1991)

T. Yokogawa, Y. Watanabe, Y. Kumazawa, T. Ueda, I. Hirao, K. Miura and K. Watanabe:
A Novel Cloverleaf Structure Found in Mammalian Mitochondrial tRNASer(UCN)
Nucleic Acids Res., 19, 6101-6105 (1991)


<1992年>

I. Kumagai, F. Sunada, S. Takeda and K. Miura:
Redesign of the Substrate-binding Site of Hen Egg White Lysozyme Based on the Molecular Evolution of C-type Lysozymes
J. Biol. Chem., 267, 4608-4612 (1992)

R. Iwase, M. Maeda, T. Fujiwara, M. Sekine, T. Hata and K. Miura:
Molecular Design of a Eukaryotic Messenger RNA and Its Chemical Synthesis
Nucleic Acids Res., 20, 1643-1648 (1992)

Y. Kumazawa, T. Yokogawa, H. Tsurui, K. Miura and K. Watanabe:
Effect of the Higher-order Structure of tRNAs on the Stability of Hybrids with Oligodeoxyribonucleotides: Separation of tRNA by an Efficient Solution Hybridization
Nucleic Acids Res., 20, 2223-2232 (1992)

Y. Takeuchi, T. Nonaka, K.T. Nakamura, S. Kojima, K. Miura and Y. Mitsui:
Crystal Structure of an Engineered Subtilisin Inhibitor Complexed with Bovine Trypsin
Proc. Natl. Acad. Sci. USA, 89, 4407-4411 (1992)

I. Kumagai, S. Takeda and K. Miura:
Functional Conversion of the Homologous Proteins α-Lactalbumin and Lysozyme by Exon Exchange
Proc. Natl. Acad. Sci. USA, 89, 5887-5891 (1992)

I. Hirao, Y. Nishimura, Y. Tagawa, K. Watanabe and K. Miura:
Extraordinarily Stable Mini-hairpins: Electrophoretical and Thermal Properties of the Various Sequence Variants of d(GCGAAAGC) and Their Effect on DNA Sequencing
Nucleic Acids Res., 20, 3891-3896 (1992)

Y. Ueda, I. Kumagai and K. Miura:
The Effects of a Unique D-loop Structure of a Minor tRNALeuUUA from Streptomyces on Its Structural Stability and Amino Acid Accepting Activity
Nucleic Acids Res., 20, 3911-3917 (1992)

Y. Ueda, S. Kojima, K. Tsumoto, S. Takeda, K. Miura and I. Kumagai:
A Protease Inhibitor Produced by Streptomyces lividans 66 Exhibits Inhibitory Activities toward Both Subtilisin BPN' and Trypsin
J. Biochem., 112, 204-211 (1992)

Y. Endo, S. Otsuzuki, M. Ito and K. Miura:
Production of an Enzymatic Active Protein Using a Continuous Flow Cell-free System
J. Biotech., 25, 221-230 (1992)

S. Taguchi, S. Kojima, I. Kumagai, H. Ogawara, K. Miura and H. Momose:
Isolation and Partial Characterization of SSI-like Protease Inhibitors from Streptomyces
FEMS Microbiol. Lett., 99, 293-298 (1992)

 

<1993年>

S. Taguchi, Y. Yoshida, I. Kumagai, K. Miura and H. Momose:
Effect of Downstream Message Secondary Structure on the Secretory Expression of the Streptomyces Subtilisin Inhibitor
FEMS Microbiol. Lett., 107, 185-190 (1993)

M. Wakiyama, I. Hirao, I. Kumagai and K. Miura:
Effect of Tandem Repeated AUG Codons on Translation Efficiency of Eukaryotic mRNA Carrying a Short Leader Sequence
Mol. Gen. Genet., 238, 59-64 (1993)

I. Kumagai, K. Maenaka, F. Sunada, S. Takeda and K. Miura:
Effects of Substrate Alterations on Substrate-binding Mode in the Active Site of Hen Egg-white Lysozyme
Eur. J. Biochem., 212, 151-156 (1993)

Y. Ueda, S. Taguchi, K. Nishiyama, I. Kumagai and K. Miura:
Effect of a Rare Leucine Codon, TTA, on Expression of a Foreign Gene in Streptomyces lividans
Biochim. Biophys. Acta, 1172, 262-266 (1993)

S. Taguchi, H. Kikuchi, S. Kojima, I. Kumagai, T. Nakase, K. Miura and H. Momose:
High Frequency of SSI-like Protease Inhibitors among Streptomyces
Biosci. Biotech. Biochem., 57, 322-524 (1993)

S. Kojima, I. Kumagai and K. Miura:
Requirement for a Disulfide Bridge near the Reactive Site of Protease Inhibitor SSI (Streptomyces Subtilisin Inhibitor) for Its Inhibitory Action
J. Mol. Biol., 230, 395-399 (1993)

S. Kojima, S. Furukubo, I. Kumagai and K. Miura:
Effects of Deletion in the Flexible Loop of the Protease Inhibitor SSI (Streptomyces Subtilisin Inhibitor) on Interactions with Proteases
Protein Engng., 6, 297-303 (1993)

I. Hirao, S. Yoshizawa and K. Miura:
Stability of mRNA in an Escherichia coli Cell-free Translation System
FEBS Lett., 321, 169-172 (1993)

M. Kitamura. K. Ozawa, S. Kojima I. Kumagai, H. Akutsu and K. Miura:
The Primary Structure of Pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) As Determined by Nucleotide Sequencing of Its Gene and Partial Amino Acid Sequencing
Protein Seq. Data Anal., 5, 193-196 (1993)

T. Kishi, K. Miura, K. Matsumoto and H. Hirokawa:
Complementation Assay of Primer Protein: Gene Expression Systems of Plasmid Vectors Support the Infection of Suppressor Sensitive Mutant Phage M2 and φ29
Jpn. J. Genet., 68, 243-255 (1993)

K. Masuda-Momma, T. Hatanaka, K. Inouye, K. Kanaori, A. Tamura, K. Akasaka, S. Kojima, I. Kumagai, K. Miura and B.Tonomura:
Interaction of Subtilisin BPN' and Recombinant Streptomyces Subtilisin Inhibitors with Substituted P1 Site Residues
J. Biochem., 114, 553-559 (1993)

T. Kumazaki, K. Kajiwara, S. Kojima, K. Miura and S. Ishii:
Interaction of Streptomyces Subtilisin Inhibitor (SSI) with Streptomyces griseus Metallo-endopeptidase II (SGMP II)
J. Biochem., 114, 570-575 (1993)

M. Matsubara, E. Kurimoto, S. Kojima, K. Miura and T. Sakai:
Quantitative In Vitro Renaturation of Subtilisin BPN' without the Aid of Pro-sequence
Chem. Lett., 1783-1786 (1993)

K. Masuda-Momma, T. Shimakawa, K. Inouye, K. Hiromi, S. Kojima, I. Kumagai, K. Miura and B. Tonomura:
Identification of Amino Acid Residues Responsible for the Changes of Absorption and Fluorescence Spectra on the Binding of Subtilisin BPN' and Streptomyces Subtilisin Inhibitor
J. Biochem., 114, 906-911 (1993)

S. Taguchi, H. Kikuchi, M. Suzuki, S. Kojima, M. Terabe, K. Miura, T. Nakase and H. Momose:
Streptomyces Subtilisin Inhibitor-like Proteins Are Distributed Widely in Streptomycetes
Appl. Environ. Microbiol., 59, 4338-4341 (1993)


<1994年>

S. Kojima, K. Fujimura, I. Kumagai and K. Miura:
Contribution of Salt Bridge in the Protease Inhibitor SSI (Streptomyces Subtilisin Inhibitor) to Its Inhibitory Action
FEBS Lett., 337, 195-199 (1994)

K. Watanabe, N. Hayashi, A. Oyama, K. Nishikawa, T. Ueda and K. Miura:
Unusual Anticodon Loop Structure Found in E. coli Lysine tRNA
Nucleic Acids Res., 22, 79-87 (1994)

S. Yoshinari, T. Koike, T. Yokogawa, K. Nishikawa, T. Ueda, K. Miura and K. Watanabe:
Existence of Nuclear-encoded 5S-rRNA in Bovine Mitochondria
FEBS Lett., 338, 137-142 (1994)

I. Hirao, G. Kawai, S. Yoshizawa, Y. Nishimura, Y. Ishido, K. Watanabe and K. Miura:
Most Compact Hairpin-turn Structure Exerted by a Short DNA Fragment, d(GCGAAGC) in Solution: An Extraordinarily Stable Structure Resistant to Nuclease and Heat
Nucleic Acids Res., 22, 576-582 (1994)

M. Kitamura, S. Kojima, K. Ogasawara, T. Nakaya, T. Sagara, K. Niki, K. Miura, H. Akutsu and I. Kumagai:
Novel FMN-binding Protein from Desulfovibrio vulgaris (Miyazaki F)-Cloning and Expression of Its Gene in Escherichia coli
J. Biol. Chem., 269, 5566-5573 (1994)

K. Akama, S. Kojima, M. Nakano, T. Tobita and H. Hayashi:
The Amino Acid Sequence and Phosphorylation Sites of a Boar Transition Protein 1
Biochem. Mol. Biol. Int., 32, 349-357 (1994)

T. Hibino, S. Misawa, M. Wakiyama, S. Maeda, K. Yazaki, I. Kumagai, T. Ooi and K. Miura:
High-level Expression of Porcine Muscle Adenylate Kinase in Escherichia coli: Effects of the Copy Number of the Gene and the Translational Initiation Signals
J. Biotech., 32 139-148 (1994)

S. Taguchi, S. Kojima, M. Terabe, K. Miura and H. Momose:
Comparative Studies on the Primary Structures and Inhibitory Properties of Subtilisin-trypsin Inhibitors from Streptomyces
Eur. J. Biochem., 220, 911-918 (1994)

M. Matsubara, E. Kurimoto, S. Kojima, K. Miura and T. Sakai:
Achievement of Renaturation of Subtilisin BPN' by a Novel Procedure Using Organic Salts and a Digestible Mutant of Streptomyces Subtilisin Inhibitor
FEBS Lett., 342, 193-196 (1994)

T. Tange, S. Taguchi, S. Kojima, K. Miura and H. Momose:
Improvement of a Useful Enzyme (Subtilisin BPN') by an Experimental Evolution System
Appl. Microbiol Biotechnol., 41, 239-244 (1994)

S. Kojima, N. Fushimi, A. Ikeda, I. Kumagai and K. Miura:
Secretory Production of Chicken Ovomucoid Domain 3 by Escherichia coli and Alteration of Inhibitory Specificity toward Proteases by Substitution of the P1 Site Residue
Gene, 143, 239-243 (1994)

S. Yoshizawa, T. Ueda, Y. Ishido, K. Miura, K. Watanabe and I. Hirao:
Nuclease Resistance of an Extraordinarily Thermostable Mini-hairpin DNA Fragment, d(GCGAAGC) and Its Application to In Vitro Protein Synthesis
Nucleic Acids Res., 22, 2217-2221 (1994)

S. Kojima, M. Terabe, S. Taguchi, H. Momose and K. Miura:
Primary Structure and Inhibitory Properties of a Proteinase Inhibitor Produced by Streptomyces cacaoi
Biochim. Biophys. Acta, 1207, 120-125 (1994)

T. Hayashi, M. Matsubara, D. Nohara, S. Kojima, K. Miura and T. Sakai:
Renaturation of the Mature Subtilisin BPN' Immobilized on Agarose Beads
FEBS Lett., 350, 109-112 (1994)

M. Terabe, S. Kojima, S. Taguchi, H. Momose and K. Miura:
Three Novel Subtilisin-trypsin Inhibitors from Streptomyces: Primary Structures and Inhibitory Properties
J. Biochem., 116, 1156-1163 (1994)

M. Terabe, S. Kojima, S. Taguchi, H. Momose and K. Miura:
Primary Structure and Inhibitory Properties of a Subtilisin-chymotrypsin Inhibitor from Streptomyces virginiae
Eur. J. Biochem., 226, 627-632 (1994)

A. Tamura, S. Kojima, K. Miura and J.M. Sturtevant:
Effect of an Intersubunit Disulfide Bond on the Stability of Streptomyces Subtilisin Inhibitor
Biochemistry, 33, 14512-14520 (1994)

T. Kishi, K. Miura, K. Matsumoto and H. Hirokawa:
Effects of Altered RGD (Arg-Gly-Asp) Sequences in the Primer Protein on the Protein Primed DNA Replication of Bacteriophage M2 In Vitro
J. Gen. Appl. Microbiol., 40, 197-208 (1994)

Y. Watanabe, G. Kawai, T. Yokogawa, N. Hayashi, Y. Kumazawa, T. Ueda, K. Nishikawa, I. Hirao, K. Miura and K. Watanabe:
Higher-order Structure of Bovine Mitochondrial tRNASerUGA: Chemical Modification and Computer Modeling
Nucleic Acids Res., 22, 5378-5384 (1994)


<1995年>

M. Wakiyama, M. Saigoh, K. Shiokawa and K. Miura:
mRNA Encoding the Translation Initiation Factor eIF-4E Is Expressed Early in Xenopus Embryogenesis
FEBS Lett. 360, 191-193 (1995)

M. Terabe, S. Kojima, S. Taguchi, H .Momose and K. Miura:
A Subtilisin Inhibitor Produced by Streptomyces bikiniensis Possesses a Glutamine Residue at Reactive Site P1
J. Biochem., 117, 609-613 (1995)

A. Tamura, S. Kojima, K. Miura and J.M. Sturtevant:
A Thermodymanic Study of Mutant Forms of Streptomyces Subtilisin Inhibitor. II. Replacement at the Interface of Dimer Formation, Val13
J. Mol. Biol., 249, 636-645 (1995)

S. Asakawa, H. Himeno, K. Miura and K. Watanabe:
Nucleotide Sequence and Gene Organization of the Starfish Asterina pectinifera Mitochondrial Genome
Genetics, 140, 1047-1060 (1995)

K. Shibuya, H. Kaji, T. Itoh, Y. Ohyama, A. Tsujigami, S. Tate, A. Takeda, I. Kumagai, I. Hirao, K. Miura, F. Inagaki and T. Samejima:
Human Cystatin A Is Inactivated by Engineered Truncation. The NH2-terminal Region of the Cysteine Proteinase Inhibitor Is Essential for Expression of Its Inhibitory Activity
Biochemistry, 34, 12185-12192 (1995)

K. Akama, H. Ichimura, H. Sato, S. Kojima, K. Miura, H. Hayashi, Y. Komatsu and M. Nakano:
The Amino Acid Sequence and Interaction with the Nucleosome Core DNA of Transition Protein 4 from Boar Late Spermatid Nuclei
Eur. J. Biochem., 233, 179-185 (1995)

S. Taguchi, M. Suzuki, S. Kojima, K. Miura and H. Momose:
Streptomyces Serine Protease (SAM-P20): Recombinant Production, Characterization, and Interaction with Endogenous Protease Inhibitor
J. Bacteriol., 177, 6638-6643 (1995)

S. Taguchi, S. Kojima, K. Miura and H. Momose:
Taxonomic Characterization of Closely Related Streptomyces Spp. Based on the Amino Acid Sequence Analysis of Protease Inhibitor Proteins
FEMS Microbiol. Lett., 135, 169-173 (1995)


<1996年>

A. Tamura, M. Matsushita, A. Naito, S. Kojima, K. Miura and K. Akasaka:
Dynamics of the Three Methionyl Side Chains of Streptomyces Subtilisin Inhibitor. Deuterium NMR Studies in Solution and in the Solid State
Protein Sci., 5, 127-139 (1996)

M. Terabe, S. Kojima, S. Taguchi, H. Momose and K. Miura:
New Subtilisin-trypsin Inhibitors Produced by Streptomyces: Primary Structures and Their Relationship to Other Proteinase Inhibitors from Streptomyces
Biochim Biophys. Acta, 1292, 233-240 (1996)

S. Kojima, Y. Kuriki, Y. Sato, F. Arisaka, I. Kumagai, S. Takahashi and K. Miura:
Synthesis of α-helix-forming Peptides by Gene Engineering Methods and Their Characterization by Circular Dichroism Spectra Measurements
Biochim. Biophys. Acta, 1294, 129-137 (1996)

S. Kojima, K. Miyoshi and K. Miura:
Synthesis of a Squash-type Protease Inhibitor by Gene Engineering and Effects of Replacements of Conserved Hydrophobic Amino Acid Residues on Its Inhibitory Activity
Protein Engng., 9, 1241-1246 (1996)


<1997年>

M. Suzuki, S. Taguchi, S. Yamada, S. Kojima, K. Miura and H. Momose:
A Novel Member of the Subtilisin-like Protease Family from Streptomyces albogriseolus
J. Bacteriol., 179, 430-438 (1997)

S. Kojima, Y. Kuriki, T. Yoshida, K. Yazaki and K. Miura:
Fibril Formation by an Amphipathic α-helix-forming Polypeptide Produced by Gene Engineering
Proc. Japan Acad., 73B, 7-11 (1997)

M. Kitamura, Y. Koshino, Y. Kamikawa, K. Kohno, S. Kojima, K. Miura, T. Sagara, H. Akutsu, I. Kumagai and T. Nakaya:
Cloning and Expression of the Rubredoxin Gene from Desulfovibrio vulgaris (Miyazaki F)-Comparison of the Primary Structure of Desulfoferrodoxin
Biochim. Biophys. Acta, 1351, 239-247 (1997)

S. Yoshizawa, G. Kawai, K. Watanabe, K. Miura and I. Hirao:
GNA Trinucleotide Loop Sequences Producing Extraordinarily Stable DNA Minihairpins
Biochemistry, 36, 4761-4767 (1997)

S. Taguchi, S. Kojima, M. Terabe, Y. Kumazawa, H. Kohriyama, M. Suzuki, K. Miura and H. Momose:
Molecular Phylogenetic Characterization of Streptomyces Protease Inhibitor Family
J. Mol. Evol., 44, 542-551 (1997)

M. Wakiyama, N. Sakai, S. Kojima and K. Miura:
Disulfide Bond Formation Is Not Involved in Cap-binding Activity of Xenopus Translation Initiation Factor eIF-4E
FEBS Lett., 409, 407-410 (1997)

S. Kojima, T. Minagawa and K. Miura:
The Propeptide of Subtilisin BPN' As a Temporary Inhibitor and Effect of an Amino Acid Replacement on Its Inhibitory Activity
FEBS Lett., 411, 128-132 (1997)

N. Nakamura, H. Taguchi, N. Ishii, M. Yoshida, M. Suzuki, I. Endo, K. Miura and M. Yohda:
Purification and Molecular Cloning of the Group II Chaperonin from the Acidothermophilic Archaeon, Sulfolobus Sp. Strain 7
Biochem. Biophys. Res. Commun., 236, 727-732 (1997)

K. Ogura, S. Tsuchiya, H. Terasawa, S. Yuzawa, H. Hatanaka, V. Mandiyan, J. Schlessinger and F. Inagaki:
Conformation of an Shc-derived Phosphotyrosine-containing Peptide Complexed with the Grb2 SH2 Domain
J. Biomol. NMR, 10, 273-278 (1997)

T. Yoshida, M. Wakiyama, K. Yazaki and K. Miura:
Transmission Electron and Atomic Force Microscopic Observation of Polysomes on Carbon-coated Grids Prepared by Surface Spreading
J. Electron Microsc., 46, 503-506 (1997)

M. Wakiyama, T. Futami and K. Miura:
Poly(A) Dependent Translation in Rabbit Reticulocyte Lysate
Biochimie, 79, 781-785 (1997)

H. Watanabe, T. Gojobori and K. Miura:
Bacterial Features in the Genome of Methanococcus jannaschii in Terms of Gene Composition and Biased Base Composition in ORFs and Their Surrounding Regions
Gene, 205, 7-18 (1997)


<1998年>

T. Yoshida, M. Yohda, M. Suzuki, K. Yazaki, K. Miura and I. Endo:
Characterization of Homo-oligomeric Complexes of α and β Chaperonin Subunits from the Acidothermophilic Archaeon, Sulfolobus Sp. Strain 7
Biochem. Biophys. Res. Commun., 242, 640-647 (1998)

S. Kojima, T. Minagawa and K. Miura:
Tertiary Structure Formation in the Propeptide of Subtilisin BPN' by Successive Amino Acid Replacements and Its Close Relation to Function
J. Mol. Biol., 277, 1007-1013 (1998)

M. Kitamura, T. Sagara, M. Taniguchi, M. Ashida, K. Ezoe, K. Kohno, S. Kojima, K. Ozawa, H. Akutsu, I. Kumagai and T. Nakaya:
Cloning and Expression of the Gene Encoding Flavodoxin from Desulfovibrio vulgaris (Miyazaki F)
J. Biochem., 123, 891-898 (1998)

S. Kojima, T. Kumazaki, S. Ishii and K. Miura:
Primary Structure of Streptomyces griseus Metalloendopeptidase II
Biosci. Biotech. Biochem., 62, 1392-1398 (1998)

M. Shibata, J. Shinga, Y. Yasuhiko, M. Kai, K. Miura, T. Shimogori, K. Kashiwagi, K. Igarashi and K. Shiokawa:
Overexpression of S-adenosylmethionine Decarboxylase (SAMDC) in Early Xenopus Embryos Induces Cell Dissociation and Inhibits Transition from the Blastula to the Gastrula Stage
Int. J. Dev. Biol., 42, 675-686 (1998)

S. Taguchi, S. Yamada, S. Kojima and H. Momose:
An Endogenous Target Protease, SAM-P26, of Streptomyces Protease Inhibitor (SSI): Primary Structure, Enzymatic Characterization, and Its Interaction with SSI
J. Biochem., 124, 804-810 (1998)

T. Tanaka, H. Matsuzawa, S. Kojima, I. Kumagai, K. Miura and T. Ohta:
P1-specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1, Using P1-substituted Derivatives of Streptomyces Subtilisin Inhibitor
Biosci. Biotech. Biochem., 62, 2035-2038 (1998)


<1999年>

D. Nohara, H. Sugiura, H. Sakakibara, M. Matsubara, S. Kojima, K. Miura and T. Sakai:
High Performance in Refolding of Streptomyces griseus Trypsin by the Aid of a Mutant of Streptomyces Subtilisin Inhibitor Designed As Trypsin Inhibitor
J. Biochem., 125, 343-347 (1999)

S. Kojima, M. Deguchi and K. Miura:
Involvement of the C-terminal Region of Yeast Proteinase B Inhibitor 2 in Its Inhibitory Action
J. Mol. Biol., 286, 775-785 (1999)

K. Ogura, S. Tsuchiya, H. Terasawa, S. Yuzawa, H. Hatanaka, V. Mandiyan, J. Schlessinger and F. Inagaki:
Solution Structure of the SH2 Domain of Grb2 Complexed with the Shc-derived Phosphotyrosine-containing Peptide
J. Mol. Biol., 289, 439-445 (1999)

G.-T. Kim, H. Tsukaya, Y. Saito and H. Uchimiya:
Changes in the Shapes of Leaves and Flowers upon Overexpression of Cytochrome P450 in Arabidopsis
Proc. Natl. Acad. Sci. USA, 96, 9433-9437 (1999)

H. Sato, K. Akama, S. Kojima, K. Miura, A. Sekine and M. Nakano:
Expression of a Zinc-binding Domain of Boar Spermatidal Transition Protein 2 in Escherichia coli
Protein Express. Purif., 16, 454-462 (1999)

H. Miyoshi, T. Youtani, H. Ide, H. Hori, K. Okamoto, M. Ishikawa, M. Wakiyama, T. Nishino, T. Ishida and K. Miura:
Binding Analysis of Xenopus laevis Translation Initiation Factor 4E (eIF-4E) in Initiation Complex Formation
J. Biochem., 126, 897-904 (1999)

S. Kojima, N. Takagi, T. Minagawa, N. Fushimi and K. Miura:
Effects of Amino Acid Replacements around the Reactive Site of Chicken Ovomucoid Domain 3 on the Inhibitory Activity toward Chymotrypsin and Trypsin
Protein Engng., 12, 857-862 (1999)


<2000年>

T. Youtani, K. Tomoo, T. Ishida, H. Miyoshi and K. Miura:
Regulation of Human eIF4E by 4E-BP1: Binding Analysis Using Surface Plasmon Resonance
IUBMB Life, 49, 27-31 (2000)

J. Yokoska, T. Tsukamoto, K. Miura, K. Shiokawa and K. Mizumoto:
Cloning and Characterization of mRNA Capping Enzyme and mRNA (Guanine-7-)-methyltransferase cDNAs from Xenopus laevis
Biochem. Biophys. Res. Commun., 268, 617-624 (2000)

K. Yazaki, T. Yoshida, M. Wakiyama and K. Miura:
Polysomes of Eukaryotic Cells Observed by Electron Microscopy
J. Electron Microsc., 49, 663-668 (2000)

E. Kajita, M. Wakiyama, K. Miura, K. Mizumoto, T. Oka, I. Komuro, T. Miyata, H. Yatsuki, H. Hori and K. Shiokawa:
Isolation and Characterization of Xenopus laevis Aldolase B cDNA and Expression Patterns of Aldolase A, B and C Genes in Adult Tissues, Oocytes and Embryos of Xenopus laevis
Biochim. Biophys. Acta, 1493, 101-118 (2000)


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