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ホーム > 研究室 > タンパク質化学(小島研究室)

タンパク質化学(小島研究室)

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2015201420132007-20122001-2006|1991-2000|

原著論文

1991

  • S. Kojima, Y. Nishiyama, I. Kumagai and K. Miura:
    Inhibition of Subtilisin BPN' by Reaction Site P1 Mutants of Streptomyces Subtilisin Inhibitor
    J. Biochem., 109, 377-382 (1991)
  • A. Tamura, K. Kanaori, S. Kojima, I. Kumagai, K. Miura and K. Akasaka:
    Mechanisms of Temporary Inhibition in Streptomyces Subtilisin Inhibitor Induced by an Amino Acid Substitution, Tryptophan 86 Replaced by Histidine
    Biochemistry, 30, 5275-5286 (1991)
  • Y. Takeuchi, S. Noguchi, Y. Satow, S. Kojima, I. Kumagai, K. Miura, K. T. Nakamura and Y. Mitsui:
    Molecular Recognition at the Active Site of Subtilisin BPN': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor SSI (Streptomyces Subtilisin Inhibitor)
    Protein Engng., 4, 501-508 (1991)

1992

  • Y. Takeuchi, T. Nonaka, K.T. Nakamura, S. Kojima, K. Miura and Y. Mitsui:
    Crystal Structure of an Engineered Subtilisin Inhibitor Complexed with Bovine Trypsin
    Proc. Natl. Acad. Sci. USA, 89, 4407-4411 (1992)
  • Y. Ueda, S. Kojima, K. Tsumoto, S. Takeda, K. Miura and I. Kumagai:
    A Protease Inhibitor Produced by Streptomyces lividans 66 Exhibits Inhibitory Activities toward Both Subtilisin BPN' and Trypsin
    J. Biochem., 112, 204-211 (1992)
  • S. Taguchi, S. Kojima, I. Kumagai, H. Ogawara, K. Miura and H. Momose:
    Isolation and Partial Characterization of SSI-like Protease Inhibitors from Streptomyces
    FEMS Microbiol. Lett., 99, 293-298 (1992)

1993

  • S. Taguchi, H. Kikuchi, S. Kojima, I. Kumagai, T. Nakase, K. Miura and H. Momose:
    High Frequency of SSI-like Protease Inhibitors among Streptomyces
    Biosci. Biotech. Biochem., 57, 322-524 (1993)
  • S. Kojima, I. Kumagai and K. Miura:
    Requirement for a Disulfide Bridge near the Reactive Site of Protease Inhibitor SSI (Streptomyces Subtilisin Inhibitor) for Its Inhibitory Action
    J. Mol. Biol., 230, 395-399 (1993)
  • S. Kojima, S. Furukubo, I. Kumagai and K. Miura:
    Effects of Deletion in the Flexible Loop of the Protease Inhibitor SSI (Streptomyces Subtilisin Inhibitor) on Interactions with Proteases
    Protein Engng., 6, 297-303 (1993)
  • M. Kitamura. K. Ozawa, S. Kojima I. Kumagai, H. Akutsu and K. Miura:
    The Primary Structure of Pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) As Determined by Nucleotide Sequencing of Its Gene and Partial Amino Acid Sequencing
    Protein Seq. Data Anal., 5, 193-196 (1993)
  • K. Masuda-Momma, T. Hatanaka, K. Inouye, K. Kanaori, A. Tamura, K. Akasaka, S. Kojima, I. Kumagai, K. Miura and B.Tonomura:
    Interaction of Subtilisin BPN' and Recombinant Streptomyces Subtilisin Inhibitors with Substituted P1 Site Residues
    J. Biochem., 114, 553-559 (1993)
  • T. Kumazaki, K. Kajiwara, S. Kojima, K. Miura and S. Ishii:
    Interaction of Streptomyces Subtilisin Inhibitor (SSI) with Streptomyces griseus Metallo-endopeptidase II (SGMP II)
    J. Biochem., 114, 570-575 (1993)
  • M. Matsubara, E. Kurimoto, S. Kojima, K. Miura and T. Sakai:
    Quantitative In Vitro Renaturation of Subtilisin BPN' without the Aid of Pro-sequence
    Chem. Lett., 1783-1786 (1993)
  • K. Masuda-Momma, T. Shimakawa, K. Inouye, K. Hiromi, S. Kojima, I. Kumagai, K. Miura and B. Tonomura:
    Identification of Amino Acid Residues Responsible for the Changes of Absorption and Fluorescence Spectra on the Binding of Subtilisin BPN' and Streptomyces Subtilisin Inhibitor
    J. Biochem., 114, 906-911 (1993)
  • S. Taguchi, H. Kikuchi, M. Suzuki, S. Kojima, M. Terabe, K. Miura, T. Nakase and H. Momose:
    Streptomyces Subtilisin Inhibitor-like Proteins Are Distributed Widely in Streptomycetes
    Appl. Environ. Microbiol., 59, 4338-4341 (1993)

1994

  • S. Kojima, K. Fujimura, I. Kumagai and K. Miura:
    Contribution of Salt Bridge in the Protease Inhibitor SSI (Streptomyces Subtilisin Inhibitor) to Its Inhibitory Action
    FEBS Lett., 337, 195-199 (1994)
  • M. Kitamura, S. Kojima, K. Ogasawara, T. Nakaya, T. Sagara, K. Niki, K. Miura, H. Akutsu and I. Kumagai:
    Novel FMN-binding Protein from Desulfovibrio vulgaris (Miyazaki F)-Cloning and Expression of Its Gene in Escherichia coli
    J. Biol. Chem., 269, 5566-5573 (1994)
  • K. Akama, S. Kojima, M. Nakano, T. Tobita and H. Hayashi:
    The Amino Acid Sequence and Phosphorylation Sites of a Boar Transition Protein 1
    Biochem. Mol. Biol. Int., 32, 349-357 (1994)
  • S. Taguchi, S. Kojima, M. Terabe, K. Miura and H. Momose:
    Comparative Studies on the Primary Structures and Inhibitory Properties of Subtilisin-trypsin Inhibitors from Streptomyces
    Eur. J. Biochem., 220, 911-918 (1994)
  • M. Matsubara, E. Kurimoto, S. Kojima, K. Miura and T. Sakai:
    Achievement of Renaturation of Subtilisin BPN' by a Novel Procedure Using Organic Salts and a Digestible Mutant of Streptomyces Subtilisin Inhibitor
    FEBS Lett., 342, 193-196 (1994)
  • T. Tange, S. Taguchi, S. Kojima, K. Miura and H. Momose:
    Improvement of a Useful Enzyme (Subtilisin BPN') by an Experimental Evolution System
    Appl. Microbiol Biotechnol., 41, 239-244 (1994)
  • S. Kojima, N. Fushimi, A. Ikeda, I. Kumagai and K. Miura:
    Secretory Production of Chicken Ovomucoid Domain 3 by Escherichia coli and Alteration of Inhibitory Specificity toward Proteases by Substitution of the P1 Site Residue
    Gene, 143, 239-243 (1994)
  • S. Kojima, M. Terabe, S. Taguchi, H. Momose and K. Miura:
    Primary Structure and Inhibitory Properties of a Proteinase Inhibitor Produced by Streptomyces cacaoi
    Biochim. Biophys. Acta, 1207, 120-125 (1994)
  • T. Hayashi, M. Matsubara, D. Nohara, S. Kojima, K. Miura and T. Sakai:
    Renaturation of the Mature Subtilisin BPN' Immobilized on Agarose Beads
    FEBS Lett., 350, 109-112 (1994)
  • M. Terabe, S. Kojima, S. Taguchi, H. Momose and K. Miura:
    Three Novel Subtilisin-trypsin Inhibitors from Streptomyces: Primary Structures and Inhibitory Properties
    J. Biochem., 116, 1156-1163 (1994)
  • M. Terabe, S. Kojima, S. Taguchi, H. Momose and K. Miura:
    Primary Structure and Inhibitory Properties of a Subtilisin-chymotrypsin Inhibitor from Streptomyces virginiae
    Eur. J. Biochem., 226, 627-632 (1994)
  • A. Tamura, S. Kojima, K. Miura and J.M. Sturtevant:
    Effect of an Intersubunit Disulfide Bond on the Stability of Streptomyces Subtilisin Inhibitor
    Biochemistry, 33, 14512-14520 (1994)

1995

  • M. Terabe, S. Kojima, S. Taguchi, H .Momose and K. Miura:
    A Subtilisin Inhibitor Produced by Streptomyces bikiniensis Possesses a Glutamine Residue at Reactive Site P1
    J. Biochem., 117, 609-613 (1995)
  • A. Tamura, S. Kojima, K. Miura and J.M. Sturtevant:
    A Thermodymanic Study of Mutant Forms of Streptomyces Subtilisin Inhibitor. II. Replacement at the Interface of Dimer Formation, Val13
    J. Mol. Biol., 249, 636-645 (1995)
  • K. Akama, H. Ichimura, H. Sato, S. Kojima, K. Miura, H. Hayashi, Y. Komatsu and M. Nakano:
    The Amino Acid Sequence and Interaction with the Nucleosome Core DNA of Transition Protein 4 from Boar Late Spermatid Nuclei
    Eur. J. Biochem., 233, 179-185 (1995)
  • S. Taguchi, M. Suzuki, S. Kojima, K. Miura and H. Momose:
    Streptomyces Serine Protease (SAM-P20): Recombinant Production, Characterization, and Interaction with Endogenous Protease Inhibitor
    J. Bacteriol., 177, 6638-6643 (1995)

1996

  • S. Taguchi, S. Kojima, K. Miura and H. Momose:
    Taxonomic Characterization of Closely Related Streptomyces Spp. Based on the Amino Acid Sequence Analysis of Protease Inhibitor Proteins
    FEMS Microbiol. Lett., 135, 169-173 (1996)
  • A. Tamura, M. Matsushita, A. Naito, S. Kojima, K. Miura and K. Akasaka:
    Dynamics of the Three Methionyl Side Chains of Streptomyces Subtilisin Inhibitor. Deuterium NMR Studies in Solution and in the Solid State
    Protein Sci., 5, 127-139 (1996)
  • M. Terabe, S. Kojima, S. Taguchi, H. Momose and K. Miura:
    New Subtilisin-trypsin Inhibitors Produced by Streptomyces: Primary Structures and Their Relationship to Other Proteinase Inhibitors from Streptomyces
    Biochim Biophys. Acta, 1292, 233-240 (1996)
  • S. Kojima, Y. Kuriki, Y. Sato, F. Arisaka, I. Kumagai, S. Takahashi and K. Miura:
    Synthesis of α-helix-forming Peptides by Gene Engineering Methods and Their Characterization by Circular Dichroism Spectra Measurements
    Biochim. Biophys. Acta, 1294, 129-137 (1996)
  • S. Kojima, K. Miyoshi and K. Miura:
    Synthesis of a Squash-type Protease Inhibitor by Gene Engineering and Effects of Replacements of Conserved Hydrophobic Amino Acid Residues on Its Inhibitory Activity
    Protein Engng., 9, 1241-1246 (1996)

1997

  • M. Suzuki, S. Taguchi, S. Yamada, S. Kojima, K. Miura and H. Momose:
    A Novel Member of the Subtilisin-like Protease Family from Streptomyces albogriseolus
    J. Bacteriol., 179, 430-438 (1997)
  • S. Kojima, Y. Kuriki, T. Yoshida, K. Yazaki and K. Miura:
    Fibril Formation by an Amphipathic α-helix-forming Polypeptide Produced by Gene Engineering
    Proc. Japan Acad., 73B, 7-11 (1997)
  • M. Kitamura, Y. Koshino, Y. Kamikawa, K. Kohno, S. Kojima, K. Miura, T. Sagara, H. Akutsu, I. Kumagai and T. Nakaya:
    Cloning and Expression of the Rubredoxin Gene from Desulfovibrio vulgaris (Miyazaki F)-Comparison of the Primary Structure of Desulfoferrodoxin
    Biochim. Biophys. Acta, 1351, 239-247 (1997)
  • S. Taguchi, S. Kojima, M. Terabe, Y. Kumazawa, H. Kohriyama, M. Suzuki, K. Miura and H. Momose:
    Molecular Phylogenetic Characterization of Streptomyces Protease Inhibitor Family
    J. Mol. Evol., 44, 542-551 (1997)
  • M. Wakiyama, N. Sakai, S. Kojima and K. Miura:
    Disulfide Bond Formation Is Not Involved in Cap-binding Activity of Xenopus Translation Initiation Factor eIF-4E
    FEBS Lett., 409, 407-410 (1997)
  • S. Kojima, T. Minagawa and K. Miura:
    The Propeptide of Subtilisin BPN' As a Temporary Inhibitor and Effect of an Amino Acid Replacement on Its Inhibitory Activity
    FEBS Lett., 411, 128-132 (1997)

1998

  • S. Kojima, T. Minagawa and K. Miura:
    Tertiary Structure Formation in the Propeptide of Subtilisin BPN' by Successive Amino Acid Replacements and Its Close Relation to Function
    J. Mol. Biol., 277, 1007-1013 (1998)
  • M. Kitamura, T. Sagara, M. Taniguchi, M. Ashida, K. Ezoe, K. Kohno, S. Kojima, K. Ozawa, H. Akutsu, I. Kumagai and T. Nakaya:
    Cloning and Expression of the Gene Encoding Flavodoxin from Desulfovibrio vulgaris (Miyazaki F)
    J. Biochem., 123, 891-898 (1998)
  • S. Kojima, T. Kumazaki, S. Ishii and K. Miura:
    Primary Structure of Streptomyces griseus Metalloendopeptidase II
    Biosci. Biotech. Biochem., 62, 1392-1398 (1998)
  • S. Taguchi, S. Yamada, S. Kojima and H. Momose:
    An Endogenous Target Protease, SAM-P26, of Streptomyces Protease Inhibitor (SSI): Primary Structure, Enzymatic Characterization, and Its Interaction with SSI
    J. Biochem., 124, 804-810 (1998)
  • T. Tanaka, H. Matsuzawa, S. Kojima, I. Kumagai, K. Miura and T. Ohta:
    P1-specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1, Using P1-substituted Derivatives of Streptomyces Subtilisin Inhibitor
    Biosci. Biotech. Biochem., 62, 2035-2038 (1998)

1999

  • D. Nohara, H. Sugiura, H. Sakakibara, M. Matsubara, S. Kojima, K. Miura and T. Sakai:
    High Performance in Refolding of Streptomyces griseus Trypsin by the Aid of a Mutant of Streptomyces Subtilisin Inhibitor Designed As Trypsin Inhibitor
    J. Biochem., 125, 343-347 (1999)
  • S. Kojima, M. Deguchi and K. Miura:
    Involvement of the C-terminal Region of Yeast Proteinase B Inhibitor 2 in Its Inhibitory Action
    J. Mol. Biol., 286, 775-785 (1999)
  • H. Sato, K. Akama, S. Kojima, K. Miura, A. Sekine and M. Nakano:
    Expression of a Zinc-binding Domain of Boar Spermatidal Transition Protein 2 in Escherichia coli
    Protein Express. Purif., 16, 454-462 (1999)
  • S. Kojima, N. Takagi, T. Minagawa, N. Fushimi and K. Miura:
    Effects of Amino Acid Replacements around the Reactive Site of Chicken Ovomucoid Domain 3 on the Inhibitory Activity toward Chymotrypsin and Trypsin
    Protein Engng., 12, 857-862 (1999)
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